Domoic acid analysis in bivalve molluscs
نویسنده
چکیده
Domoic acid (DA) was identified as the toxin responsible for an outbreak of illness in Canada in 1987, caused by eating blue mussels that had accumulated DA as a result of the presence of Pseudo-nitzschia pungens. It is named amnesic shellfish poisoning (ASP). Since a regulatory level of 20 mg DA/kg of shellfish meat was established. The methods of determination of domoic acid are reviewed: biological assays in vivo and in vitro, biochemical and chemical assays. The AOAC mouse bioassay, which is used for PSP, cannot detect domoic acid at the regulatory level of 20 mg/kg tissue and no interlaboratory validation of this method for domoic acid has been carried out. The Quillian method, a liquid chromatography-UV detection method is approved as European Norm by CEN and recommended by Codex Alimentarius as reference method. The European Commission Regulation stipulate that the reference method shall be the HPLC method and the Community Reference Laboratory on Marine Biotoxins (CRLMB) recommends Quillian method as routine method. Others protocols such as receptor binding assay, immunoassays and thin layer chromatography can be used as alternative for routine monitoring. The liquid chromatography-mass spectrometry method allows to confirm the presence of domoic acid isomers.
منابع مشابه
Monitoring the Presence of Domoic Acid in the Production Areas of Bivalve Molluscs
Algal biotoxins, chemical compounds produced by some microscopic algae, constitute the phytoplankton. The mussels, feeding on phytoplankton, can accumulate these compounds to become themselves toxic. There have been several cases of food poisoning by consumption of contaminated shellfish. Such food poisoning have pushed our health care system to provide monitoring of shellfish in the framework ...
متن کاملMechanisms contributing to low domoic acid uptake by oysters feeding on Pseudo-nitzschia cells. I. Filtration and pseudofeces production
Bivalve molluscs feeding on toxigenic Pseudo-nitzschia spp. are the main vector of domoic acid (DA) to humans. Although different oyster species rarely exceed the internationally adopted regulatory level for shellfish harvesting closures (20 μg g–1), these are often applied to all bivalve species in an affected area. This study examines the influence of diet composition and Pseudo-nitzschia mul...
متن کاملDistribution of Molluscs in the Eastern Persian Gulf, PG-GOOS Cruise
Distribution of molluscs in the eastern part of the Persian Gulf were studied during the PG-GOOS cruise (Oct-Nov 2012), using the 250 cm2 Van Veen grab for sampling. Sixty gastropod and 23 bivalve taxa in addition to one scaphopod taxon were identified. Gastropods constituted 80% of molluscs. The abundance of molluscs averaged 1398.36 (±261.95) ind./m2. Costellaria sp. was the most abundant ...
متن کاملCommittee on Toxicity of Chemicals in Food Consumer Products and the Environment Statement on Amnesic Shellfish Poisoning
1. We have been asked by the Food Standards Agency to review the issue of amnesic shellfish poisoning (ASP). The Committee was asked to consider whether the current EU action limit for bivalve shellfish (e.g. mussels, scallops, clams) of 20μg domoic acid/g tissue is adequate for the protection of public health. The Committee was also asked to comment on the public health implications of a propo...
متن کاملAppearance and spread of diseases among bivalve molluscs in the northern hemisphere in relation to international trade.
Bivalve mollusc culture is a well-developed marine aquaculture activity in many countries around the world, notably in the northern hemisphere. During the development of this activity, numerous countries have been confronted with infectious diseases of varying severity and duration. Research has been conducted to determine the aetiology, epidemiology and control measures for these epizootics. M...
متن کامل